一、团队名称

基因组稳定性调控和癌症治疗团队

二、团队组成

团队共4人，副教授2人，讲师1人，兼职教授1人

三、团队介绍

团队研究方向：

1、DNA损伤应答及真核细胞基因组稳定性维持的细胞生物学及生物化学基础；

2、肿瘤细胞转移的表观遗传学机制；

3、恶性肿瘤临床检测标志物及新的抗肿瘤药物靶点的开发。

主要研究内容包括

1、DNA损伤修复蛋白53BP1参与的细胞自噬调控机制；

2、DNA损伤修复蛋白53BP1通过相分离稳定异染色质成分的机制研究；

3、表观遗传学参与的肿瘤细胞转移的机制研究。

四、科研成果

（1）代表性的科研项目：

1．国家自然科学基金：泛素交联酶UbcH7在DNA损伤应答中的功能和机制分析（31501150）（张雷主持）                       

2．湖北省武汉市晨光计划：一种以UbcH7为靶点的对抗肿瘤放化疗耐受的短肽药物的研发（2016070204010140） （张雷主持）       

3. 武汉东湖高新区第九批“3551光谷人才计划” 多肽类抗肿瘤放化疗辅助联用药物“优必希”的研发。（张雷主持）

4. 国家级大学生创新创业训练计划“多种恶性肿瘤早期诊断试剂盒的研发” （201710500013）（张雷主持）

5. 国家重点研发计划、人内源性逆转录病毒的相关研究，2017.07-2020.06，（陈明月主持）

（2）代表性论文（SCI）： 

1. Lei Zhang, Jinshan Tang, Fangfang Wang, Yu Ichida, Arishya Sharma, Franklin Mayca Pozo, Xinsheng Yao, and Youwei Zhang,* “53BP1 is required for the protective effect of autophagy independent of DSB repair. “ MOL CELL, Major Revision

2. Lei Zhang, Jing Yu, Liujun Chen, Shangrong Wu,Zhongpei Zhang, Youwei Zhang “Prior infection with common respiratory virus  is a risk factor for Coronavirus disease 2019 (COVID-19) through increasing ACE2 expression” Nature Microbiology, Under review.

3. #Xiangzi Han, #Lei Zhang , #Jinsil Chung, Franklin Mayca Pozo, Amanda Tran, Darcie D. Seachrist, James W. Jacobberger,　Ruth A. Keri, Hannah Gilmore,  Youwei zhang. “UbcH7 regulates 53BP1 stability and DSB repair”, Proc Natl Acad Sci U S A. 111(49):17456–17461, 2014 Dec 9.（Co-First Author）

4，He R, Xie X, Lv L, Huang Y, Xia X, Chen X, *Zhang L. “Comprehensive investigation of aberrant microRNAs expression in cells culture model of MnCl2-induced neurodegenerative disease.” Biochem Biophys Res Commun 2017 Apr 29;486(2):342-348. doi: 10.1016/j.bbrc.2017.03.041.

5. Qing G, Lu Q, Xiong Y, Zhang L, Wang H, Li X, Liang X, Sun T. “New Opportunities and Challenges of Smart Polymers in Post-Translational Modification Proteomics.” Adv Mater, 2017 May;29(20). doi: 10.1002/adma.201604670. Epub 2017 Jan 23.

6. Yifeng Gu, Xiaowu Chen, *Lei Zhang, “Differential expression analysis of Paralichthys olivaceus microRNAs in adult ovary and testis by deep sequencing”. General and Comparative Endocrinolog, 204(1) :181-184,2014 Aug.

7. GOLT1A-KISS1 Fusion Is Associated With Metastasis in Adenoid Cystic Carcinomas. Lei Zhang , Anyi Cheng , Yanan Yu , Nengxing Zou , Wenxiu Wang , Linyue Lv , Xiaoxia Guo , Mingyue Chen , Youwei Zhang Biochem Biophys Res Commun ,2020 Mar 17

8. Lv L.Y., Chen W.R., Cheng A.Y., Yu Y.N., Wen C., *Zhang L. Aberrant glycosylation in a metastatic cell model of salivary adenoid cystic carcinoma, Basic & Clinical Pharmacology & Toxicology, 121 (Suppl. 5), 1–38

9. Lv L.Y., Wen C., Chen W.R., Cheng A.Y., Yu Y.N., *Zhang L. Identification of metastasis-associated gene fusions in salivary adenoid cystic carcinoma. Basic & Clinical Pharmacology & Toxicology, 121 (Suppl. 5), 1–38

10. Bing Xu*, Xiaoxia Guo*, Sumi Mathew, Angel L Armesilla, James Cassidy, John L Darling, Weiguang Wang, Triptolide simultaneously induces reactive oxygen species, inhibits NF-κB activity and sensitizes 5-fluorouracil in colorectal cancer cell lines. Cancer Letters 2010; 291: 200-8 (*joint first authors)  

11. Xiaoxia Guo, Shuchita Pandey, Bing Xu, Weiguang Wang, Disulfiram/copper complex inhibiting NF-κB activity and potentiating cytotoxic effect of gemcitabine on colon and breast cancer cell lines. Cancer Letters 2010; 290: 104-13  

12. Xiaoxia Guo, Elisabeth Goessl, Elaina S.R. Collie-Duguid, James Cassidy, Vincent O’brien. Cell Cycle Perturbation and Acquired 5-Fluoracil Chemoresistance. Anticancer Research 2008; 28: 9-14

13. Xiaoxia Guo, Jeff Evans, Angel Armesilla, John Darling, Andreas Schatzlein, James Cassidy, Weiguang Wang. In vitro evaluation of cancer specific NF-κB-CEA enhancer-promoter system for gene-directed enzyme prodrug therapy in colon cancer cell lines. British Journal of  Cancer 2007; 97: 745-54

14. Helene K Myrvang, Xiaoxia Guo, Chan Li and Lodewijk V Dekker. Protein interactions between surface Annexin A2 and S100A10 mediate adhesion of breast cancer cells to .17microvascular endothelial cells. FEBS 2013; 587(19),3210-3215

15. Mingyue Chen,Jie Cui，Discovery of endogenous retroviruses with mammalian envelopes in avian genomes uncovers long-term bird-mammal interaction，Virology，2019，Academic Press Inc. 0042-6822

16. Hongbo Wang,Yingchao Zhao, *Mingyue Chen, *Jie Cui。 Identification of Novel Long Non-coding and Circular RNAs in Human Papillomavirus-Mediated Cervical Cancer，Frontiers in Microbiology，2017，Frontiers Media S.A. 1664-302X；

17. #Xiaoman Wei,#Mingyue Chen, Jie Cui， Bayesian evolutionary analysis for emerging infectious disease: an exemplified application for H7N9 avian influenza viruses，，Science China Life Sciences，2017，， 1674-7305；

18. Du Y, Chen M, Yang J, Jia Y, Han S, Holmes EC, Cui J. Molecular evolution and emergence of H5N6 avian influenza virus in central China， Journal of Virology，2017，American Society for Microbiology，0022-538X。

五、研究生毕业去向

多名已毕业研究生进入武汉大学医学研究院，中科院武汉病毒所等单位进行博士研究生学习或进入国内知名生物医药企业就业，本团队积极鼓励和推荐学生出国交流和深造。

六、联系方式

联系人： 张雷

邮箱：zhanglei@hbut.edu